Analysis of the Complete Genome Sequence of Bacillus atrophaeus GQJK17 Reveals Its Biocontrol Characteristics as a Plant Growth-Promoting Rhizobacterium

Figure 3

In vitro antagonistic activities of B. atrophaeus GQJK17 against F. solani (a), B. subtilis (b), and E. coli (c). The antifungal activity of GQJK17 was tested against F. solani. Newly cultivated hyphal plugs of F. solani were placed on the center of a PDA plate and incubated for 1 day at 28°C. Then, strain GQJK17 was inoculated onto one side of the plug at a distance of 2 cm and incubated for another 3 days. The antibacterial assays of GQJK17 were performed against E. coli and B. subtilis. The precultured E. coli or B. subtilis was incubated in 5 mL of LB liquid medium for 10 h at 37°C. Then, 1 mL of the culture was mixed with 100 mL of LB semisolid medium. Strain GQJK17 was inoculated on the center of the plate and incubated for 1 day at 28°C.

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