Tsantan Sumtang Alleviates Chronic Hypoxia-Induced Pulmonary Hypertension by Inhibiting Proliferation of Pulmonary Vascular Cells

Figure 6

Effects of Tsantan Sumtang on Cyclin D1 and CDK4 protein expression in lung tissue of HPH rats (n=5). Ctr: control group; Hyp: untreated hypoxia group (rats exposed to hypoxia in hypobaric chamber, equal to the parameter in altitude 4500 m), and Hyp+SW-treated groups (rats exposed to hypoxia in hypobaric chamber and received increasing doses of Tsantan Sumtang treatment, 1.0 g/kg, 1.25 g/kg, and 1.5 g/kg). Upper panel: relative expression levels of Cyclin D1 (a) and CDK4 (b) protein (optical density of Cyclin D1 and CDK4 normalized against β-actin expression). Lower panel: β-actin protein expression was used as control. (a) Cyclin D1 expression was significantly higher in Hyp group and Tsantan Sumtang treatment of all 3 doses significantly downregulated protein expression of Cyclin D1 in HPH rats with the best effect of high dose (^ΔP< 0.05 compared to Hyp; < 0.05 compared to Ctr); (b) CDK4 expression was meaningfully higher in Hyp group and Tsantan Sumtang treatment of 1.25 g/kg and 1.5 g/kg doses considerably downregulated protein expression of CDK4 in HPH rats, and the 1.5 g/kg Tsantan Sumtang treatment group showed best effect with no difference with the control rats on CDK4 expression (^ΔP< 0.05 compared to Hyp; < 0.05 compared to Ctr).

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