Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori

Figure 1

Alignment of the amino acid sequences of HpEno with homologs from different biological species. The species include H. pylori 26695 (P48285), H. cetorum (I0ELQ0), X. fastidiosa (Q9PDT8), S. pneumoniae (Q97QS2), E.coli (P0A6P9)and E. histolytica (N9UZM6), P. falciparum (Q27727), T. brucei (Q38BV6), S. cerevisiae (P00924), and human (α-enolase, P06733). The red signals under the letters represent rectangle, metal binding site reported only for humanα-enolase; open circle, substrate binding sites; star, active site residues; triangle, metal binding sites; close circle, subtract binding site in inhibited form. The black lines indicate the sites for RNA-binding and the dashed lines regions for plasminogen binding in S. pneumonia. The yellow line corresponds to loop L1, pink line to loop L2, and green line to loop L3, blue line corresponds to signature of enolase.