Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori

Figure 2

SDS-PAGE electrophoresis of expression and purification of HpEno. (a) Overexpression of HpEno in E.coli strain Rosetta-gami, lane 1: prestained protein marker (7-175 kDa), lane 2: induction with IPTG (), lane 3: induction for 1 hour (T₁), lane 4: induction for 2 hours (T₂), lane 5: induction for 3 hours (T₃), lane 6: induction for 4 hours (T₄), and lane 7: induction overnight (). (b) Affinity chromatography and His-tag removal, lane 1: prestained protein marker (7-175 kDa), lane 2: overexpression of HpEno, lane 3: solubilization of inclusion bodies, lane 4: flow through, lane 5: his-tag cut with PSP after 18 hr, lane 6: his-tag cut with PSP after 24 hr, lane 7: enolase no-cleaved, abd lane 8: HpEno cleaved.

(a)

(b)