Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori

Figure 6

Enzymatic Activity of HpEno. Black circle: ScEno TEA buffer with MgSO₄ (25 mM); red triangle: HpEno in tris-acetate buffer with MgSO₄ (5 mM); green triangle: HpEno in TEA buffer with MgSO₄ (5 mM); blue triangle: HpEno in HEPES buffer with MgSO₄ (0.5 mM); cyan triangle: HpEno in TEA buffer with MgSO₄ (0.5 mM); yellow triangle: HpEno with the His-tag in TEA buffer with MgSO₄ (25 mM); pink triangle: crude extract from inclusion bodies in TEA buffer with MgSO₄ (5 mM); orange triangle: HpEno in TEA buffer with MgSO₄ (25 mM).