BioMed Research International

Research Article

Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori

Table 1

Amino acids involved in enzymatic activity and biological functions of enolases.


Feature	*S. cerevisiae*	*H. sapiens*	*E. histolytica*	*S. pneumoniae*	*H. pylori*	*X. fastidiosa*

Mg²⁺	D²⁴⁷ E²⁹⁶ D³²¹	S⁴⁰ D²⁴⁵ E²⁹³ D³¹⁸		D²⁴² E²⁹¹ D³¹⁸	D²⁴² E²⁸⁶ D³¹³	D²⁴⁴ E²⁸⁷ D³¹⁴

Catalytic sites	H¹⁶⁰ E¹⁶⁹ E²¹² E²⁹⁶ D³²¹ K³⁴⁶ R³⁷⁵ S³⁷⁶ K³⁹⁷	H¹⁵⁸ E¹⁶⁷ E²¹⁰ E²⁹³ D³¹⁸ K³⁴³ R³⁷² S³⁷³ K³⁹⁴	H¹⁵⁶ E¹⁶⁵ E²⁰⁸ E²⁹⁶ D³²² K³⁴⁷ R³⁷⁶ S³⁷⁷ K³⁹⁸	H¹⁵⁵ E¹⁶⁴ E²⁰⁵ E²⁹¹ D³¹⁸ K³⁴³ R³⁷² S³⁷³ K³⁹⁴	H¹⁵⁵ E¹⁶⁴ E²⁰⁵ E²⁸⁶ D³¹³ K³³⁸ R³⁶⁷ S³⁶⁸ K³⁸⁹	H¹⁵⁷ E¹⁶⁶ E²⁰⁷ E²⁸⁷ D³¹⁴ K³³⁹ R³⁶⁸ S³⁶⁹ K³⁹⁰

Loops of active site	³⁷SGASTGVH⁴⁴ ¹⁵⁹SHAGGALA¹⁶⁶ ²⁵⁰SSEFFKDGKYDLD FKNPESDKSKWL²⁷⁴	³⁷SGASTGIY⁴⁴ ¹⁵⁷SHAGNKLA¹⁶⁴ ²⁴⁸ASEFFRSGKYDLD FKSPDDPSRYI²⁷¹	³⁷SGASTGVH⁴⁴ ¹⁵⁵AHAGNALA¹⁶² ²⁴⁶ASEFYNEETKKYD LGKKIPADKKDPSLVK²⁷⁴	³⁹SGASTGEH⁴⁶ ¹⁵⁴SHSDAPIA¹⁶¹ ²⁴⁵SSEFYDKERKVYD YTKFEGEGAAVR²⁶⁹	³⁹SGASTGKR⁴⁶ ¹⁵⁴THANNSID¹⁶¹ ²⁴⁵SSELVDENFNYHL KGENKIL²⁶⁴	³⁹SGASTGTK⁴⁶ ¹⁵⁶AHADNNVD¹⁶³ ²⁴⁷SSEFRDNGKYNLV GENKRL²⁶⁶

RNA binding	⁵⁶KWMGKGVLH⁶⁴ ¹⁰³KSKLGA¹⁰⁸ ¹⁹²HNLKSLTKKRYGA SAGN²⁰⁸ ³¹⁵GIQI³¹⁸ ⁴⁰³RSERLAK⁴⁰⁹ ⁴³⁵DKL⁴³⁷	⁵⁶RYMGKGVSK⁶⁴ ¹⁰³KSKFGA¹⁰⁸ ¹⁹⁰HNLKNVIKEKYGKD ATN²⁰⁶ ³¹²GIQV³¹⁵ ⁴⁰⁰RSERLAK⁴⁰⁶ ⁴³²LAK⁴³⁴	⁵⁶RYGGKGVLK⁶⁴ ¹⁰¹KGKLGA¹⁰⁶ ¹⁸⁸QCLKVVIKAKYGQ DATN²⁰⁴ ³¹⁵GNFQI³¹⁹ ⁴⁰⁴RSERLCK⁴¹⁰ ⁴³⁵TA⁴³⁶	⁵⁸RYGGLGTQK⁶⁶ ¹⁰³KGKLGA¹⁰⁸ ¹⁸⁷HALKKILKSRGLET A²⁰¹ ³¹⁰GKKVQL³¹⁵ ⁴⁰⁰RTDRIAK⁴⁰⁶ ⁴³²LKK⁴³⁴	⁵⁸RFLGKGVLR⁶⁶ ¹⁰³YANLGA¹⁰⁸ ¹⁸⁷HTLKKLLDGKNQLT²⁰⁰ ³⁰⁵GRQIQL³¹⁰ ³⁹⁵RSERIAK⁴⁰¹ ⁴²⁰G⁴²⁰	⁵⁸RYLGKGVRA⁶⁶ ¹⁰³KGRLGA¹⁰⁸ ¹⁸⁹HALKSVLKGQGLST²⁰² ³⁰⁶GHKVQL³¹¹ ³⁹⁶RSDRVAK⁴⁰² ⁴²⁸LKS⁴³⁰

Enolase signature	³⁴³LLLKVNQIGTLSES³⁵⁶	³⁴⁰LLLKVNQIGSVTES³⁵³	³⁴⁴VLIKVNQIGTLTET³⁵⁷	³⁴⁰ILIKVNQIGTLTET³⁵³	³³⁵VLIKPNQIGTISET³⁴⁸	³³⁶ILIKLNQIGTLTET³⁴⁹

Hydrophobic domain	³³SIVPSGAST⁴¹	³³AAVPSGAST⁴¹	³³SCVPSGAST⁴¹	³⁵GMVPSGAST⁴³	³⁵AIVPSGAST⁴³	³⁵AAVPSGAST⁴³

Plasminogen binding	²⁵³FFKDGKY²⁵⁹	²⁵¹FFRSGKY²⁵⁷	²⁴⁹FYNEETKKY²⁵⁷	²⁴⁸FYDKERKVY²⁵⁶	²⁴⁸LVDENFNY²⁵⁵	²⁵⁰FRDNGKY²⁵⁶