Biochemical and Biophysical Characterization of the Enolase from<i> Helicobacter pylori</i>

<table class="fixed-width table-group" id="tab2"><tr><td><table class="table"><colgroup><col style="width:5.50em"/><col style="width:5.62em"/><col style="width:10.30em"/><col style="width:7.64em"/></colgroup><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left"><b>Organism</b></td><td class="align_center"><b>Condition</b></td><td class="align_center"><b>Proposed mechanism</b></td><td class="align_center"><span style="width: 25.0981ptpx;"><svg height="11.3124pt" id="M5" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.2626 25.0981 11.3124" width="25.0981pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M622 0V29L350 665L310 655L39 29V0H622ZM462 58H119L290 487L462 58Z"></path></g><g transform="matrix(.013,0,0,-0.013,8.638,0)"><path d="M746 650H445V615C520 608 528 602 528 517V370H255V517C255 602 263 608 335 615V650H35V615C112 608 121 602 121 517V133C121 48 112 42 39 35V0H337V35C263 42 255 48 255 133V319H528V133C528 48 520 42 441 35V0H746V35C670 42 662 48 662 132V517C662 602 668 608 746 615V650Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,24.462,-5.741)"><path d="M-164 587L-241 611L-454 -75L-377 -99L-164 587Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,18.817,-5.741)"><path d="M566 317V388H52V317H566ZM566 126V197H52V126H566Z"></path></g></svg><b>(kJ mol</b><sup><b>−1</b></sup><b>)</b></span></td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left"><b><i>H. pylori</i></b></td><td class="align_center">+Mg<sup>2+</sup></td><td class="align_center"><span style="width: 50.5212ptpx;"><svg height="19.9154pt" id="M6" style="vertical-align:-3.181499pt" version="1.1" viewbox="-0.0498162 -16.7339 50.5212 19.9154" width="50.5212pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 85C108 42 83 30 29 28L23 0H260L266 28C193 33 173 42 176 89C178 122 186 172 202 255L256 527H259L583 -8H612L690 390C708 481 720 535 728 558C744 603 756 619 816 622L822 650Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,10.062,3.132)"><path d="M414 144C384 79 371 75 317 75H135L276 221C367 316 408 376 408 465C408 570 327 635 237 635C179 635 131 609 100 575L42 494L67 471C94 510 138 565 205 565C277 565 321 517 321 435C321 348 258 270 195 195C146 137 88 81 33 26V0H411C423 44 433 88 446 135L414 144Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,21.47,-8.782)"><path d="M485 418C485 434 469 451 441 451C389 451 312 387 255 335C221 304 194 278 159 242H157L259 678C264 698 264 710 255 710C242 710 183 681 96 673L90 643L128 642C165 641 171 640 162 602L24 -5L31 -12C51 -4 81 4 110 9L145 181C154 195 180 220 205 242C233 170 263 106 291 53C318 1 335 -12 360 -12C383 -12 425 0 483 82L463 105C437 79 411 57 400 57C388 57 378 68 355 110C329 156 288 244 268 298C299 333 355 376 405 376C426 376 440 373 448 368C453 365 465 368 468 373C477 386 485 405 485 418Z"></path></g><g transform="matrix(.013,0,0,-0.013,18.64,0)"><path d="M567 230V280H69V230H567Z"></path></g><g transform="matrix(.013,0,0,-0.013,18.014,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z"></path></g><g transform="matrix(.013,0,0,-0.013,34.125,0)"><path d="M412 140C382 77 369 73 315 73H129L270 222C362 320 402 379 402 466C402 571 322 635 234 635C177 635 130 609 99 576L42 495L64 475C90 514 133 568 201 568C274 568 318 519 318 435C318 349 255 267 193 193C144 135 87 78 32 23V0H405C417 45 427 89 440 131L412 140Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.365,0)"><path d="M743 375C743 570 607 650 407 650H136L130 622C214 612 220 606 204 526L125 122C110 43 105 36 24 27L17 0H250C382 0 482 23 571 76C680 141 743 248 743 375ZM644 379C644 188 520 36 305 36C277 36 249 38 229 46C205 55 202 71 212 126L293 553C300 588 306 600 317 606C330 613 356 617 390 617C547 617 644 537 644 379Z"></path></g></svg></span></td><td class="align_center">179 (8)</td></tr><tr><td class="align_left" colspan="4"><hr/></td></tr><tr><td class="align_left"><b>Yeast</b></td><td class="align_center">+Mg<sup>2+</sup></td><td class="align_center"><span style="width: 50.5212ptpx;"><svg height="19.9154pt" id="M7" style="vertical-align:-3.181499pt" version="1.1" viewbox="-0.0498162 -16.7339 50.5212 19.9154" width="50.5212pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 85C108 42 83 30 29 28L23 0H260L266 28C193 33 173 42 176 89C178 122 186 172 202 255L256 527H259L583 -8H612L690 390C708 481 720 535 728 558C744 603 756 619 816 622L822 650Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,10.062,3.132)"><path d="M414 144C384 79 371 75 317 75H135L276 221C367 316 408 376 408 465C408 570 327 635 237 635C179 635 131 609 100 575L42 494L67 471C94 510 138 565 205 565C277 565 321 517 321 435C321 348 258 270 195 195C146 137 88 81 33 26V0H411C423 44 433 88 446 135L414 144Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,21.47,-8.782)"><path d="M485 418C485 434 469 451 441 451C389 451 312 387 255 335C221 304 194 278 159 242H157L259 678C264 698 264 710 255 710C242 710 183 681 96 673L90 643L128 642C165 641 171 640 162 602L24 -5L31 -12C51 -4 81 4 110 9L145 181C154 195 180 220 205 242C233 170 263 106 291 53C318 1 335 -12 360 -12C383 -12 425 0 483 82L463 105C437 79 411 57 400 57C388 57 378 68 355 110C329 156 288 244 268 298C299 333 355 376 405 376C426 376 440 373 448 368C453 365 465 368 468 373C477 386 485 405 485 418Z"></path></g><g transform="matrix(.013,0,0,-0.013,18.64,0)"><path d="M567 230V280H69V230H567Z"></path></g><g transform="matrix(.013,0,0,-0.013,18.014,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z"></path></g><g transform="matrix(.013,0,0,-0.013,34.125,0)"><path d="M412 140C382 77 369 73 315 73H129L270 222C362 320 402 379 402 466C402 571 322 635 234 635C177 635 130 609 99 576L42 495L64 475C90 514 133 568 201 568C274 568 318 519 318 435C318 349 255 267 193 193C144 135 87 78 32 23V0H405C417 45 427 89 440 131L412 140Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.365,0)"><path d="M743 375C743 570 607 650 407 650H136L130 622C214 612 220 606 204 526L125 122C110 43 105 36 24 27L17 0H250C382 0 482 23 571 76C680 141 743 248 743 375ZM644 379C644 188 520 36 305 36C277 36 249 38 229 46C205 55 202 71 212 126L293 553C300 588 306 600 317 606C330 613 356 617 390 617C547 617 644 537 644 379Z"></path></g></svg></span></td><td class="align_center">190 (30)</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Activation enthalpies associated to the thermal unfolding reaction of enolases.</div>

BioMed Research International

tab2

Table 2

Table 2: Biochemical and Biophysical Characterization of the Enolase from<i> Helicobacter pylori</i> 

BioMed Research International

Biochemical and Biophysical Characterization of the Enolase from Helicobacter pylori

Table 2