Research Article
Genetic Heterogeneity of Alicyclobacillus Strains Revealed by RFLP Analysis of vdc Region and rpoB Gene
Table 2
List of isolates and their RFLP profiles. AAT, A. acidoterrestris; AAC, A. acidocaldarius; BA, Brevibacillus agri; BG, bacillus ginsengihumi.
| | Isolate | Source | Guaiacol production | Erythritol utilization | RFLP analysis | 16S/
Hin61 | vdc/
BsuRI | vdc/
Hin61 | vdc/
HphI | rpoB/
BsuRI | rpoB/
Hin61 | rpoB/
HphI |
| | 1 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 2 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 3 | intermediate for beverage production | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 4 | intermediate for beverage production | no | no | AAC | - | - | - | AAC(I) | AAC(I) | AAC(I) | | 5 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 6 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 7 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 8 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 9 | orange beverage | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 10 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 11 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 12 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 13 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 14 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 15 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 16 | concentrated apple juice | yes | non typical | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 17 | banana nectar | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 18 | orange juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 19 | concentrated orange juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 20 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 21 | orange juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 22 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 23 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 24 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 25 | fresh apples | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 26 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 27 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 28 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 29 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 30 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 31 | concentrated black currant juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 32 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 33 | concentrated apple juice | yes | non typical | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 34 | fresh apples | yes | yes | AAT | AAT(I) | AAT(I) | AAT(IA) | AAT(I) | AAT(I) | AAT(I) | | 35 | fresh apples | | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 36 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 37 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 38 | intermediate for beverage production | no | no | AAC | - | - | - | AAC(I) | AAC(I) | AAC(IA) | | 39 | concentrated apple juice | yes | - | BG | - | - | - | BG | BG | BG | | 40 | concentrated apple juice | yes | - | BG | - | - | - | BG | BG | BG | | 41 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 42 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(IIA) | | 43 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 44 | spoiled apple beverage | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 45 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 46 | spoiled apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 47 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 48 | concentrated strawberry juice | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) | | 49 | concentrated cherry juice | no | no | AAC | - | - | - | AAC(IA) | AAC(I) | AAC(IA) | | 50 | tomato juice from fresh tomatoes | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) | | 51 | concentrated beetroot juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 52 | concentrated cherry juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 53 | concentrated cherry juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 54 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(IIA) | AAT(II) | AAT(II) | | 55 | concentrated cherry juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 56 | concentrated raspberry juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 57 | concentrated apple juice | yes | non typical | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 58 | orange juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 59 | concentrated apple juice | no | non typical | AAC | - | - | - | AAC(IA) | AAC(I) | AAC(I) | | 60 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 61 | concentrated apple juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 62 | tomato juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 63 | concentrated apple juice | yes | - | BA | - | - | - | BA | BA | BA | | 64 | concentrated apple juice | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) | | 65 | cherry puree | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 66 | concentrated black currant juice | yes | yes | AAT | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | AAT(I) | | 67 | concentrated strawberry juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 68 | cloudy apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 69 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 70 | concentrated apple juice | no | no | AAC | - | - | - | AAC(IA) | AAC(I) | AAC(I) | | 71 | concentrated apple juice | no | no | AAC | - | - | - | AAC(IA) | AAC(I) | AAC(I) | | 72 | concentrated apple juice | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) | | 73 | concentrated apple juice | yes | yes | AAT | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | AAT(II) | | 74 | concentrated apple juice | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) | | 75 | concentrated apple juice | no | no | AAC | - | - | - | AAC(II) | AAC(II) | AAC(II) |
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