Targeting N-Terminal Huntingtin with a Dual-sgRNA Strategy by CRISPR/Cas9

Figure 1

Capability of initiating translation by noncanonical in-frame ATGs. (a) Candidate in-frame ATGs before exon 7 of HTT. (b) Constructs of HTT fragments fused with GFP. (c, d) Sanger sequencing results of junctions in plasmid constructs. (e) Transfection of constructs in HEK293 cells. Scale bar, 100 μm. (f) Western blot analysis of fused proteins. Asterisks represented the E3a-ATG initiated translation products.

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