GAS1 Deficient Enhances UPR Activity in Saccharomyces cerevisiae

Figure 2

GAS1 deficiency enhances the UPR activity in yeast cells. (a) HAC1 mRNA splicing in the BY4742, gas1Δ, and GAS1 OE strains. HAC1u and HAC1i indicate the unspliced and spliced HAC1 mRNA, respectively. (b) Relative expression of UPR target genes, including ERO1, LHS1, KAR2, FKB2, EUG1, and PDI1 in the BY4742, ire1Δ, gas1Δ, and GAS1 OE strains. The data represent the means ± S.D. (n = 6). p < 0.01 vs BY4742; p < 0.01 vs BY4742. (c) Relative expression of Kar2p in the BY4742, gas1Δ, and GAS1 OE strains determined by western blotting, with G6PDH used as an internal control. BY4742 was considered to be the control.

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