GAS1 Deficient Enhances UPR Activity in Saccharomyces cerevisiae

Figure 5

No obvious differences in UPR activity between the GAS1-deficient and wild-type strains under the tunicamycin-induced stress. (a) HAC1 mRNA splicing in the mutant strains treated with or without 1 μg/mL Tm for 1 h. HAC1u and HAC1i indicate the unspliced and spliced HAC1 mRNA, respectively. (b) Relative expression of UPR target genes, such as EUG1, ERO1, LHS1, KAR2, FKB2, and PDI1 in the BY4742, gas1Δ, and GAS1 OE strains. The data represent the means ± S.D. (n = 4). p < 0.01 vs BY4742; p < 0.01 vs BY4742. BY4742 was considered to be the control.

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