Bax Targeted by miR-29a Regulates Chondrocyte Apoptosis in Osteoarthritis

Figure 2

Upregulation of Bax in IL-1β induced chondrocyte-like ATDC5 cell apoptosis. ATDC5 cell was cultured and supplemented with ITS for two weeks. Then cells were treated with 1, 5, 10, and 20 ng/ml IL-1β for 24 h. Cells were stained with Hoechst 33258 for 10 min and the representative images were shown in (a). The apoptotic rate was counted and shown in (b) and donates p < 0.05, compared to 0 group. Cells treated with 10 ng/ml IL-1β for 6, 12, 24, and 48 h and representative images were shown in (c) and statistical data were shown in (d) and donates p < 0.05, compared to 0 group. (e) Lysate of cell treated in (c) was subjected to western blot with Bax, PUMA, Bcl-2, Cytochrome c (Cyto c), and active caspase-3 antibodies. Tubulin was stained with loading control. The relative expression levels were shown in (f). , #, and & donate p < 0.05, compared to 0 group.

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