Bax Targeted by miR-29a Regulates Chondrocyte Apoptosis in Osteoarthritis

Figure 4

miR-29a/Bax axis contribute to IL-1β induced chondrocyte-like ATDC5 cell apoptosis. Cells were transfected with dose-dependent of miR-29a mimic (a) or inhibitor (b). The response levels of miR-29a were determined. (c) Representative immunoblots show Bax, PUMA, and cleaved Caspase-3 levels in IL-1β induced chondrocyte-like ATDC5 cell with or without miR-29a mimic or inhibitor. (d) The relative expression levels of Bax were shown. donates p < 0.05, compared with expression level under IL-1β treatment in control group. (e) Cell apoptosis was determined by Hoechst staining in cells transfected with or without miR-29a mimic/inhibitor under IL-1β treatment. The apoptotic rates were shown in (f). (g) Graphic abstract showing the summary of the hypothesis.

(a)

(b)

(c)

(d)

(e)

(f)

(g)