MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7
Figure 1
The hypertrophic myocardium and cardiomyocytes miR-195-3p/-5p expression. (a) The effects from different concentration of Ang II on ANP, BNP mRNA in H9c2 cells. (b) Representative images of H9c2 cardiomyocytes treated with Ang II or without (control) Ang II (scale bar: 50 μm). (c) Tail-cuff method was used to monitor blood pressure at baseline and 14 days after starting the angiotensin II infusion or sham surgery. MAP: mean arterial pressure. (d) The change of gross morphology of a mouse model heart in hypertrophy induced by AngII-infusion. (e) Comparison of heart-to-body ratio between the Ang II infusion models (n = 5) and sham mice (n = 5). (f) Histological analysis of the heart tissue from different groups using H&E staining (50μm). (g) ANP and BNP mRNA expression in heart tissue were measured by the qRT-PCR assay. (h) The echocardiography results in two-week Ang-II infusion models and the sham group (n = 5). (i) Expressed miR-195-5p together with miR-195-3p were detected by RT-PCR in AngII-induced cardiomyocyte hypertrophy. (j) In hypertrophic heart tissue, miR-195-5p together with miR-195-3p were determined by qRT-PCR. The data is obtained as the mean ± SD; , p < 0.05; #, p<0.05 compared to all other groups; n= 5 or 3 different cultures.