MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7

Figure 4

Targeting of miR-195-5p to the 3′-UTR of MFN2 and FBXW7. (a) The rat miR-195-5p sequence alignment with the MFN2 and FBXW7 3′-UTR region. (b, c) The qRT-PCR analyses of miR-195-5p mimic/inhibitor transfected MFN2 and FBXW7. (d-f) The MFN2 and FBXW7 levels of protein expression in H9C2 cells under different conditions. (g, h) The H9c2 cardiomyocytes were transfected with reporter plasmid and mimic of miR-195-5p/NC-mimic for 2 days. Relative luciferase intensity was determined with a luciferase reporter assay. The data is shown as the mean ± SD; , p < 0.05, n = 3.

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