Knockdown of PHF5A Inhibits Migration and Invasion of HCC Cells via Downregulating NF-κB Signaling

Figure 4

PHF5A is involved in HCC progression via the NF-κB signaling. (a) The luciferase reporter assay showed that transactivity of NF-κB signaling is significantly inhibited in PHF5A-silenced cells. (b) The expression of p65 in cell nucleus is significantly decreased in PHF5A-silenced cells by western blotting, and p84 serves as the nucleus loading control. (c) The expressions of p-IKK-β, IKK-β, p-IκBα, and IκBα in total lysate of cells by western blotting assay, and α-tubulin serves as the loading control. (d) Relative mRNA expression of MMP9 and Slug is significantly decreased in PHF5A-silenced HCC cells by qPCR assay. (e) The expression of MMP9 and Slug is dramatically decreased in PHF5A-silenced HCC cells by western blotting assay. (f) Wound healing assay showed that blocking NF-κB pathway can inhibit the stimulative effect of PHF5A on migration of HCC cells using PDTC. (g) Transwell matrix penetration assay showed that blocking NF-κB pathway can inhibit the stimulative effect of PHF5A on invasion of HCC cells using PDTC. P < 0.05.

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