Microenvironment of Mycobacterium smegmatis Culture to Induce Cholesterol Consumption Does Cell Wall Remodeling and Enables the Formation of Granuloma-Like Structures

Figure 2

Minimal medium inducing Mycobacterium smegmatis to use cholesterol. Bacteria cells in the early stationary phase of growth in Middlebrook 7H9 broth or minimal medium (MM), with or without glycerol and/or cholesterol, were assayed by spectrofluorimetry after filipin staining. Culture groups: Middlebrook 7H9 broth (7H9+Gly) and minimal medium (MM) with or without glycerol and/or cholesterol (MM+Gly; MM+Gly+Chol; MM+Chol; and MM). p < 0.05 compared to 7H9+Gly group. This experiment was performed in triplicate, and the values are plotted as means ± SD.