Neonatal Streptococcus pneumoniae Pneumonia Induces an Aberrant Airway Smooth Muscle Phenotype and AHR in Mice Model

Figure 2

Neonatal S. pneumoniae pneumonia alters airway smooth muscle productions in mice model. IHC staining of lung tissues from both mock-infected (control) and neonatal S. pneumoniae pneumonia mice (S.pp) showing the in situ expression of α-smooth muscle actin (α-SMA) (a), smooth muscle myosin heavy chain (SMMHC) (f), and smooth muscle 22 alpha (SM22α) (k) (400x magnification). The relative α-SMA-positive (α-SMA⁺ area/Pbm²) (b), SMMHC-positive (SMMHC⁺ area/Pbm²) (g), and SM22α-positive (SM22α⁺ area/Pbm²) (l) areas are also shown. RT-qPCR was used to analyze Acta2-mRNA (c), Myh11-mRNA (h), and Tagln-mRNA (m) levels in the lung tissues. The α-SMA (d, e), SMMHC (i, j), and SM22α (n, o) protein levels in the lung tissues were analyzed by Western blotting. All data are presented as means ± SD. (n =5/group). P<0.01, compared to the control group.

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