YAP Levels Combined with Plasma CEA Levels Are Prognostic Biomarkers for Early-Clinical-Stage Patients of Colorectal Cancer

Figure 1

YAP expression in fresh colorectal cancer specimens. (a) Representative western blot analyses of YAP expression in tumor tissues T and the adjacent normal mucosae N. β-Actin was used as the control. (b) The relative YAP protein levels in 30 matched pairs of CRC tissues and the adjacent normal mucosae were calculated using the CRC tissue of sample 1 as the reference. Data are expressed as mean ± standard deviation (SD). The mean YAP protein level in the tumor tissues was significantly increased () compared with that in the adjacent normal mucosae. (c) Representative quantitative real-time polymerase chain reaction (qPCR) of YAP mRNA expression in tumor tissues and the adjacent normal mucosae. β-Actin was used as the control. (d) The relative YAP mRNA levels in 30 matched pairs of CRC tissues and the adjacent normal mucosae were calculated using the adjacent normal mucosa of sample 1 as the reference. Data are expressed as mean ± SD. The mean fold changes of YAP in the tumors clearly increased compared with those in the adjacent normal mucosae ().

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