[Retracted] miR-509-5p Inhibits the Proliferation and Invasion of Osteosarcoma by Targeting TRIB2

Figure 4

p21 may be the downstream effect of miR-509-5p targeting TRIB2. (a) Luciferase reporter gene assay was conducted to assess whether miR-509-5p directly targeted TRIB2 in cells cotransfected with pMir-Wt or pMir-Mut and either miR-509-5p overexpression vector or NC-miR. The mean of the results from cells transfected with expression control vector and pMir-con was set as 100. vs. pMir-Wt. (b) The silence effect was detected by western blot after U2OS and HOS cell lines were transfected with si-TRIB2. (c, d) Cell proliferation and invasion ability of U2OS and HOS cells transfected with si-TRIB2 or si-NC were detected by CCK-8 or Transwell assay, respectively. (e) The p21 expressions of U2OS and HOS cells transfected with si-TRIB2 or si-NC were detected by western blot. (f) Luciferase reporter gene assay showed that a markedly enhanced luciferase activity was observed in the cells cotransfected with si-TRIB2 and p21 mutant version (p21-luc).

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