Research Article
The Indirect ELISA Trypanosoma evansi in Equids: Optimisation and Application to a Serological Survey including Racing Horses, in Thailand
Table 1
Summary of data from 2 outbreaks of surra in horses in Thailand.
| | Location | Surat Thani | Ratchaburi |
| | Starting date | April 2011 | August 2011 |
| | Total number of horses in the farm | 12 | 17 | |
| | Positive rates (n/N) at t0 | Stained blood smear | 27% (3/11) | ND | | HCT | 36% (4/11) | 40% (6/15) | | CATT | 45% (5/11) | 53% (8/15) | | ELISA | 55% (6/11) | 80% (12/15) | | PCR | 55% (6/11) | 73% (11/15) |
| | Positive rates at t0 + # months | CATT | t0 + 4 months, 67% (8/12) | t0 + 1 month, 64% (7/11) | | ELISA | 92% (11/12) | 81% (9/11) | | HCT | 92% (11/12) | 36% (4/11) |
| | Percentage of horses exhibiting clinical signs (n/N) | 92% (11/12) | 87% (13/15) |
| | Percentage of horses exhibiting nervous signs (n/N) | 0% (0/12) | 13% (2/15) |
| | Treatments applied | DA | 3.5 mg/kg and 7 mg/kg | 3.5 mg/kg and 7 mg/kg | | MH | 0.25 mg/kg to 4 horses | 0.5 mg/kg + fly-proof stables for 4 horses |
| | Morbidity rate (n/N) | 92% (11/12) | 88% (15/17) |
| | Mortality rate (n/N) | 92% (11/12) | 53% (9/17) |
| | Lethality rate (n/N) | 100% (11/11) | 60% (9/15) |
| | Recovery rate (n/N) | 0% (0/11) | 40% (6/15) |
| | Remarks | Relapse of parasites in blood and clinical signs observed in most of the horses, 2-3 weeks after each treatment | 1 out of 4 horses exhibited nervous signs before MH treatment; QSC 5 mg/kg used as curative and preventive treatment |
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n = number of animals positive; N = number of animals tested or observed; ND = not done.
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