Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells

Figure 1

Evaluation of cytotoxicity on anti-inflammatory effects and nitrite oxide production in mouse RAW 264 cells pretreated with several algal ethanol extracts. RAW264 cells were treated with algal ethanol extracts at concentrations of 1/5000, 1/1000, 1/500, 1/100, and 1/50 of Aurantiochytrium 4W-1b (a), Botryococcus braunii BOT-22 (b), Euglena gracilis EOD-1 (c), and Parietichytrium sarkarianum 6b-10F (d) for 24 h. After treatment, cell proliferation was measured by MTT assay. Values are expressed as the mean ± SD for triplicate experiments and are expressed as a percentage of the control. (e) Cells were treated with algal ethanol extracts at concentrations of 1/5000 or 1/1000 for 24 h. After treatment, cells were activated with LPS (1 ng/mL) for 12 h. The amount of NO production was measured by Griess reaction. Values are expressed as the mean ± SD of triplicate experiments and are expressed as a percentage of the control LPS (+). The asterisk indicates a mean value that is significantly different from that of the control group ( p < 0.01).

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