Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells

Figure 3

Effects of pretreatment with AL4W-1b extract on gene expression of TNF-α, IL-1β, and IL-6 in RAW264 cells. RAW264 cells were pretreated with or without 1/1000 AL4W-1b for 24 h. After pretreatment, the cells were activated with LPS (1 ng/mL) for 3 h. After treatment, the gene expression levels of TNF-α, IL-1β, and IL-6 were evaluated using RT-PCR. Values are expressed as the mean ± SD of triplicate experiments. The asterisks indicate a mean value that is significantly different from that of the control group ( p < 0.01).