Anti-Inflammatory Effects of Aurantiochytrium limacinum 4W-1b Ethanol Extract on Murine Macrophage RAW264 Cells

Figure 5

Reduction in nitrite oxide production in mouse RAW 264 cells pretreated with an ethanol extract of AL4W-1b and an inhibitor of the NF-κB pathway, BAY 11-7082. RAW264 cells were pretreated with AL4W-1b extracts at a concentration of 1/1000 for 24 h. After treatment, the cells were treated with or without 10 μM BAY11-7082 (BAY) for 30 min, and then cells were activated with LPS (1 ng/mL) for 12 h. The amount of NO produced was measured by the Griess reaction. Values are expressed as the mean ± SD of triplicate experiments and are expressed as a percentage of the control LPS (+) treatment. The asterisks indicate a mean value that is significantly different from that of the control group ( p < 0.01).