Atorvastatin Inhibits Breast Cancer Cells by Downregulating PTEN/AKT Pathway via Promoting Ras Homolog Family Member B (RhoB)

Figure 2

RhoB is significantly downregulated in human breast cancer tissues. (a-b) Transcriptome profiling data from MDA-MB-231 treated with Fluvastatin or mock-treated control cells was downloaded from GSE33552. (a) Heat map and list of the differentially expressed genes of statins-treated cells and mock-treated cells (). (b) Volcano map of the differentially expressed genes of the two groups (). (c-e) RhoB expression in breast cancer patients. Data was downloaded from TCGA and determined using the Metabolic gEne RApid Visualizer. (c) RhoB is significantly downregulated in primary tumor comparing with normal tissue. (d) Compared with ER-negative patients, the RhoB mRNA expression levels of ER-positive patients were significantly increased. (e) The expression of RhoB was lower in luminal B, HER2-enriched and basal-like subtypes when compared with luminal A and basal-like subtype being the lowest. (f) Kaplan-Meier relapse-free survival (RFS) curves of RhoB (n = 3951, P = 7E-06 by log-rank test for significance). Data were analyzed using the Kaplan-Meier Plotter. (g) RhoB mRNA and protein expression were downregulated in breast cancer tissues than paired normal breast tissues which was analyzed by RT-qPCR and Western blotting. Values represent the mean ± SD from three independent measurements. p < 0.05.

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