Atorvastatin Inhibits Breast Cancer Cells by Downregulating PTEN/AKT Pathway via Promoting Ras Homolog Family Member B (RhoB)

Figure 5

Knockdown of RhoB promotes MCF-7 cells migration, proliferation, and EMT and upregulates PTEN/AKT pathway. (a) MCF-7 cells were transfected with small interfering RNA of RhoB (siRhoB1,2,3) or negative control (si-NC) and detected by RT-qPCR and Western blotting. Si-RhoB2 was chosen for the further experiment. (b) Knockdown of RhoB enhances the proliferation of MCF-7 cells detected by the CCK-8 assay. (c) RhoB knockdown upregulates the proliferation of MCF-7 cells detected by the colon formation assay. (d) Wound healing assay reveals that RhoB knockdown enhances the ability of migration of MCF-7 cells. (e) RhoB knockdown enhances the migration ability of MCF-7 cells revealed by transwell assay. (f) The effect of transfecting with si-RhoB or si-NC on the protein levels of RhoB, PTEN, p-AKT, AKT, E-cadherin, vimentin, and snail in MCF-7 cells. Values represent the mean ± SD from three independent measurements. p < 0.05.

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