Atorvastatin Inhibits Breast Cancer Cells by Downregulating PTEN/AKT Pathway via Promoting Ras Homolog Family Member B (RhoB)

Figure 6

Overexpression of RhoB inhibits MDA-MB-231 cells migration, proliferation, and EMT and downregulates PTEN/AKT pathway. (a) MDA-MB-231 cells were transfected with flag-PAK7 overexpression plasmid or flag-NC negative control vector and detected by RT- qPCR and Western blotting. (b) Overexpression of RhoB inhibits the proliferation of MDA-MB-231 cells which was detected by the CCK-8 assay. (c) RhoB overexpression downregulates the proliferation of MDA-MB-231 cells detected by the colon formation assay. (d) Wound healing assay reveals that RhoB overexpression reduces the ability of migration of MDA-MB-231 cells. (e) RhoB overexpression suppresses the migration of MDA-MB-231 cells revealed by transwell assay. (f) The effect of transfecting with flag-RhoB overexpression plasmid or flag-NC negative control vector on the protein levels of RhoB, PTEN, p-AKT, AKT, E-cadherin, vimentin, and snail in MDA-MB-231 cells. (g) Working model for the regulation of PTEN/AKT pathway suppressed by atorvastatin via upregulating the expression of RhoB. Values represent the mean ± SD from three independent measurements. p < 0.05.

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