Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property

Figure 3

Real-time quantitative PCR verification of the specific functional genes identified by DNA microarray and IPA. (a) Three basic biofunction related genes filtrated by IPA. After DNA microarray, the whole data were input into ingenuity pathway analysis system. The genes classified to antioxidant genes, inflammatory genes, and metabolic regulating genes are displayed with more than twofold expression change by myricetin. (b) Real-time PCR verification data versus microarray data. HepG2 cells were pretreated with or without myricetin for 9 h. RNA extract and real-time PCR were described in Material and Methods. The result was expressed as the relative expression level. Each value represents the mean ± SD of three separate experiments, p < 0.05; p < 0.01 versus control, respectively. CTL, control; AKR1C, aldo-keto reductase family 1, member C; GCLC, glutamate cysteine ligase, catalytic subunit; SERPINE, serpin peptidase inhibitor clade; IL, interleukin; IGFBP1, insulin -like growth factor-binding protein 1.

(a)

(b)