Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property

Figure 4

Influence of myricetin on the production of inflammatory proteins and cytokines. (a) Influence of myricetin on the production of iNOS and COX-2 protein. HepG2 cells (1×106 cells) were precultured for 24 h and starved in serum-free medium for 2.5 h. The cells were then treated with the indicated concentrations of myricetin for 30 min and then exposed to 1 μg/mL LPS for 12 h. The proteins of iNOS, COX-2, and α-tubulin were detected by Western blotting with their antibodies, respectively. (b) Myricetin decreased the levels of multiple inflammatory cytokines in HepG2 cells. The levels of 27 kinds of cytokines were measured by multiplex technology and bio-plex assay and arranged in an order from high to low change in the experimental inflammatory HepG2 cells. The data represent mean ± SD of four mice. p < 0.05 and p < 0.01. CTL, control; IL, interleukin; IFN-γ, interferon-gamma; TNF-α, tumor necrosis factor.

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