Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2

<div>BCRP-mediated uptake of riboflavin into<i> Sf9</i> inside-out membrane vesicles. Each data point represents mean ± SD from 3-4 determinations, expressed as pmol riboflavin/mg protein. (a) Time course of riboflavin (10 <i>μ</i>M) uptake into<i> Sf9</i> membrane vesicles overexpressing BCRP/ABCG2 (filled circles) and EV vesicles (open circles). Protein amount is 10 <i>μ</i>g; (b) effect of sucrose (osmolarity) on ATP-dependent uptake of riboflavin (10 <i>μ</i>M) into<i> Sf9 </i>vesicles. The transport was determined after 5 min in the presence of varying sucrose concentrations (250-1000<i>μ</i>M).</div>

BioMed Research International

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Figure 1

Figure 1: Peroxynitrite and 4-Hydroxynonenal Inactivate Breast Cancer Resistance Protein/ABCG2 