MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

Figure 1

Expression of PTHrP in human normal and OA chondrocytes. Human knee articular cartilage was taken from subjects after total knee arthroplasty or amputation surgery. (a) Tissue morphology and Safranin O/Fast Green staining of normal and OA articular cartilage (scale bars, 100 μm). (b) RT-PCR was used to detect the relative levels of PTHrP in normal and OA chondrocytes, p<0.05, n(normal) = 8, and n(OA) = 16. (c) Toluidine blue staining and immunohistochemical staining of chondrocytes (scale bars, 100 μm). The number of PTHrP (+) cells after IHC is presented as mean ± SD in the lower panels (p<0.01, n = 6). (d) Western blot analysis shows the relative expression of PTHrP in chondrocytes. The quantitative results are presented as mean ± SD in the lower panels (p<0.05, n = 4).

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