MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

Figure 2

Effect of PTHrP on chondrocyte proliferation and differentiation. OA chondrocytes were transfected with siPTHrP or a control plasmid or treated with rhPTHrP. (a) Cell viability was tested with a CCK8 kit after 24, 48, 72, 96, and 120 h. (b) qRT-PCR was performed to examine the MMP-13, collagen II, and aggrecan expression in the chondrocytes (p<0.05, p<0.001 in comparison to the control, n = 6). (c) Calcium deposition was visualised by Alizarin Red S staining (scale bars, 100 μm). Calcium deposition (+) cells are presented as mean ± SD in the lower panels (p<0.05, p<0.01, and n = 6).

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