MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

Figure 3

PTHrP is a direct target of miR-15a-5p. (a) Sequences of miR-15a-5p and potential miR-15a-5p-binding sites in the 3′UTR of PTHrP. (b) Luciferase activity assay for pGL3-PTHrP-wt or pGL3-PTHrP-mut versus the activity of Renilla luciferase in HEK293T cells after transient transfection with miR-NC or miR-15a-5p mimics (p<0.001). (c) Level of PTHrP in OA chondrocytes treated with miR-15a-5p-mimics or anti-miR-15a-5p by western blot (p<0.001). (d, e) RT-PCR analysis of the relative expression of miR-15a-5p or PTHrP (p<0.05, p<0.001, n(normal) = 8, and n(OA) = 16). (f) Correlation between miR-15a-5p and PTHrP in OA chondrocytes.