BioMed Research International

Research Article

MicroRNA-15a-5p Regulates the Development of Osteoarthritis by Targeting PTHrP in Chondrocytes

Table 2

Primer sequences used in this study.
Name 5′-3′ sequence
PTHrP plasmidF:CTAGCATTTTGTAAATGTATCTTGGTGCTGCTGAATTTCT
ATATTTTTTGTAACATAATGCACTTTAGATATACATATCAA
GT
 R:CTAGACTTGATATGTATATCTAAAGTGCATTATGTTACAA
AAAATATAGAAATTCAGCAGCACCAAGATACATTTACAAAA
TG
PTHrP mutant plasmidF:CTAGCATTTTGTAAATGTATCTTGGACGACGAGAATTTCT
ATATTTTTTGTAACATAATGCACTTTAGATATACATATCAA
GT
 R:CTAGACTTGATATGTATATCTAAAGTGCATTATGTTACAA
AAAATATAGAAATTCTCGTCGTCCAAGATACATTTACAAAA
TG
siPTHrPF: GGUGGAGACGUACAAAGAGTT
 R: CUCUUUGUACGUCUCCACCTT
miR-15a-5p (mimics)F: UAGCAGCACAUAAUGGUUUGUG
 R: CAAACCAUUAUGUGCUGCUAUU
miR-15a-5p (control)F: UUCUCCGAACGUGUCACGUTT
 R: ACGUGACACGUUCGGAGAATT
miR-15a-5p (inhibitors)F: CACAAACCAUUAUGUGCUGCUA
β-ActinF: CACCCAGCACAATGAAGATCAAGAT
 R: CCAGTTTTTAAATCCTGAGTCAAGC
PTHrPF: CTGGTTCAGCAGTGGAGCGT
 R: AAGGAAGAATCGTCGCCGTA
U6F: CTCGCTTCGGCAGCACA
miR-15a-5pF: GCGTAGCAGCACATAATGGTTTGTG
Collagen IIF: CAGGACCAAAGGGACAGAAAGG
 R: GCAAAGTTTCCACCAAGACCAG
AggrecanF: AGCTCTGGGGAGGAATCTGG
 R: GCAGTTCACCAACCGTAGGAGT
MMP-13F: GCCTTCAAAGTTTGGTCCGATG
 R: TGGTCAAGACCTAAGGAGTGGC