Investigation of Interaction between Vitamin D Receptor Gene Polymorphisms and Environmental Factors in Early Childhood Caries in Chinese Children

Table 1

Primers and MGB probes for VDR gene polymorphisms.

Candidate gene	Primer	Fragment length (bp)	MGB probe	Probe direction
FokI (rs10735810)	5′-CTGGCACTGACTCTGGCTCT-3′	88 bp	5′-FAM-TTGCCTCC[A]TCCCT-3′MGB	Reverse
	5′-GGGTCAGGCAGGGAAGTG-3′		5′-VIC-TTGCCTCC[G]TCCCT-3′MGB
TaqI (rs731236)	5′-TTCTCTATCCCCGTGCCC-3′	97bP	5′-FAM-CGCTGAT[C]GAGGC-3′MGB	Forward
	5′- TGTACGTCTGCAGTGTGTTGG- 3′		5′-VIC-CGCTGAT[T]GAGGCC-3′MGB
Bsm I (rs1544410)	5′-GGATTCTGAGGAACTAGATAAGCA 3′	91bP	5′-FAM-CCTGC[A]CATTCC-3′MGB	Forward
	5′-CCAGTTCACGCAAGAGCAG-3′		5′-VIC-CCTGC[G]CATTCC-3′MGB
ApaI (rs7975232)	5′-GGCACGGGGATAGAGAAGA-3′	96 bp	5′-FAM-CTGGGC[A]CCTCAC-3′MGB	Forward
	5′-GCTGCCGTTGAGTGTCTGT-3′		5′-VIC-CTGGGC[C]CCTCAC-3′MGB
CdX-2 (rs11568820)	5′-AGAACATCTTTTGTATCAGGAACTT-3′	98 bp	5′-FAM-CTAGGTCACA[A]TAAAA-3′MGB	Forward
	5′-TTCAAAATTTTAACTGCAACCC-3′		5′-VIC-CTAGGTCACA[G]TAAAA-3′MGB