Research Article

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Figure 4

SOD1, GPx1, and p53 localization in lizard liver. SOD1 (a, b) and GPx1 (c, d) localization. In untreated lizards SOD1 (a) and GPx1 (c) are present in hepatocytes cytoplasm; in 0.05 μg/kg bw Gly-treated lizards, SOD1 (b) and GPx1 (d) are detectable in the hepatocytes nuclei (↑) as well as in the cytoplasm. (e, f) p53 detection. The protein is absent in untreated animals (e) and detectable in the Kupffer cells (dotted arrow) of 0.05 μg/kg bw Gly-treated lizards (f). (D’): negative control of reaction. Proteins localization does not change in 0.5 μg/kg bw Gly-treated lizards (data not shown). The bar is 30 μm.

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