mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells
Table 2
Content of 24 types of intracellular fatty acid in control cells and RICTOR-silencing cells (%).
Fatty acid
Contro
shRICTO
Butyric acid (4:0)
0.81%
0.57% (p=1.00)
Lauric acid (12:0)
0.19%
0.12% (p=1.00)
Myristic acid (14:0)
1.14%
0.28% (p=0.22)
Pentadecanoic acid (15:0)
0.54%
0.08% (p=0.25)
cris-10-Pentadecenoic acid (15:1)
3.04%
0.68% (p=0.01)
Palmitic acid (16:0)
10.33%
5.62% (p=0.01)
Heptadecanoic acid (17:0)
1.04%
0.39% (p=0.45)
Stearic acid (18:0)
32.60%
19.82% (p=0.0001)
Linolelaidic acid (18:2n6t)
2.05%
1.15% (p=0.30)
Linoleic acid (18:2n6c)
5.39%
3.95% (p=0.36)
cis-11-Eicosenoic acid (20:1n9)
3.89%
2.03% (p=0.13)
cis-11,14,17-Eicosatrienoic acid (20:3n3)
3.38%
1.71% (p=0.15)
Eicosapentaenoic acid (20:5n3)
3.44%
2.37% (p=0.44)
Behenic acid (22:0)
0.95%
0.68% (p=0.73)
Erucic acid (22:1n9)
8.79%
1.93% (p=0.0001)
cis-13,16-Docosadienoic acid (22:2)
3.51%
2.61% (p=0.45)
Docosahexaenoic acid (22:6n3)
4.17%
2.22% (p=0.14)
Lignoceric acid (24:0)
0.85%
0.50% (p=0.69)
cris-10- Heptadecenoic acid (17:1)
3.28%
4.49% (p=0.41)
cis-11,14-Eicosadienoic acid (20:2)
2.41%
4.31% (p=0.14)
Arachidonic acid (20:4n6)
4.23%
10.39% (p=0.0001)
Henicosanoic acid (21:0)
0.66%
0.84% (p=1.00)
Tricosanoic acid (23:0)
0.57%
0.73% (p=1.00)
Nervonic acid (24:1n9)
2.76%
3.44% (p=0.71)
Total
100%
70.93% (p=0.0001)
a: content of product in control group/total content in control group × 100%. b: content of product in RICTOR-silencing group/total content in control group × 100%. shRICTOR: RICTOR-silencing (p<0.01).
