The Alcohol Extract of Coreopsis tinctoria Nutt Ameliorates Diabetes and Diabetic Nephropathy in db/db Mice through miR-192/miR-200b and PTEN/AKT and ZEB2/ECM Pathways

Figure 4

Effects of AC on target gene expression and the PI3K/AKT signaling pathway. Western blotting was used to detect protein expression. (a) Effect of AC on the protein expression levels of ZEB2 and COL4 α1 in mouse kidneys. (b) Grey level analysis of ZEB2 and COL4 α1 bands. (c) Effect of AC on the protein expression levels of PTEN, PI3K p85α, P-AKT/AKT, and P-smad3/smad3 in mouse kidneys. (d) Grey level analysis of PTEN, PI3K p85α, P-AKT/AKT, and P-smad3/smad3 bands. : P<0.05 compared with the NC group; #: P<0.05 compared with the DN group, one-way analysis of variance.

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