Hepatitis B Virus X Protein-Induced RORγ Expression to Promote the Migration and Proliferation of Hepatocellular Carcinoma

Figure 5

HBx can increase the promoter activity of RORγ. (a) HepG2 and (b) Huh7 cells were transfected with pGL3-RORγ, complemented with HBx plasmid. After 24 h transfection, the cells were subjected to analyze the reporter activity with the luciferase reporter system. (c) The stale overexpressing HBx cells and the vector controls of HepG2 and (d) Huh7 cells were transfected with pGL3-RORγ reporter plasmid and Rellina plasmid, and the luciferase activity was detected by the dual-luciferase reporter system. (e) The knockdown efficiency of HBx was assessed in the HBx-stable overexpressing cells of HepG2 and Huh7. (f) The stable HBx-overexpressing HepG2 and (g) Huh.7 cells were transfected with pGL3-RORγ reporter plasmid and cotransfected with siRNA against HBx. With 24 h culture, the promoter activity of RORγ was detected by the luciferase reporter value. The data are expressed as mean ± standard deviation (SD), n = 4. , were considered statistically significant.

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