Hepatitis B Virus X Protein-Induced RORγ Expression to Promote the Migration and Proliferation of Hepatocellular Carcinoma

Figure 6

RORγ promotes the migration activity of HepG2 cells. (a) The expression of HepG2, Huh7, SMMC7721, and HCCLM3 cell lines was evaluated with RT-PCR. (b) The relative quantitative mRNA levels of RORγ were analyzed with Quantity One. (c) HepG2 cells were transfected with lentiviral particles of RORγ and control vector, and with 24 h culture, the cells were subjected to RT-PCR to confirm the efficiency of overexpression of RORγ. (d) HepG2 cells were transfected with lentiviral particles, and further cultured for indicated times, the convergence of cells were recorded. (e) The migration index was calculated with the ratio of the scratch area between RORγ overexpression and vector cells by Image J software. The data are expressed as mean ± standard deviation (SD), n = 3. , , were considered statistically significant.

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