Effects of Different Methionine Sources on Methionine Metabolism in the IPEC-J2 Cells

Table 1

Selected genes and primers used in this study.

Gene	Primer sequences	Product size (bp)	Tm (°C)
β-actin	F: CCAGGTCATCACCATCGG	158	60
	R: CCGTGTTGGCGTAGAGGT
MAT-2A	F:CACTTTGCCTTGGTTACGCC	85	52
	R:TCTGATGGGAAGCACAGCAC
AHcy	F: CGGACACTTTGACGTGGAGA	93	62
	R: AACAAGTAGCGGTCCACCTG
CBS	F: TGCTCACTACGACATCACAGC	127	60
	R: GCACTTCTCCTTCAGCTTCCT
CTH	F: GGTTCCAACATTTCGCCACG	138	61
	R: ACTCAAAACCCGAGTGCTGT
MTHFR	F:AGACCATACTGCACATGACCTG	155	61
	R:GTAGCTGAAGCCTCCTTCCTC
MTR	F:TTGGAGGATGCTGTGGTA	159	55
	R:TAACGAAGTTGGTGTATGGT
DAO	F:GATGCCCCTTGGCTGAAGAA	173	62
	R:CAGCCTTCCCAGATGGTGTT
HADH	F: GCCATCGTGGAGAACCTGAA	159	62
	R:GAAATGGAGCCCGGCAAATC
HAO1	F:CGGAATGTGGCTGAAGTAGACC	136	62
	R:TCCTACAGGCTCTCACGGTTG
APN	F: CAATATGCCGCCCAAAGGTTC	163	61
	R: CCGGATCAGGACGCCATTT
MetAP-1	F:GATTTGTGAAGGCGGATGGC	132	63
	R:CGGGTTAGGATCTCACAGCC
MetAP-2	F:CATGCTGGGAAAACAGTGCC	108	61
	R: AACAATGCCTTTGCCTGTGC