Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis

Figure 3

Reversal of enhanced migration and invasion of osteosarcoma cells under hypoxic conditions by knocking down HMGN5. (a) Wound-healing assay in 24 hours and 48 hours for each group. (b, c) Statistical analysis of cell migration rate of each group in 24 hours and 48 hours; the results showed that the migration ability of the HIF1A^OE-GFP group was significantly enhanced compared with the Cherry-GFP group, while the migration ability of the HIF1A^OE-HMGN5^sh group was significantly lower than that of HIF1A^OE-GFP group. (d) Transwell invasion assay for each group. (e) Statistical analysis of invading cells of each group; the results showed that the invasion ability of the HIF1A^OE-GFP group was significantly enhanced compared with the Cherry-GFP group, while the invasion ability of the HIF1A^OE-HMGN5^sh group was significantly lower than that of the HIF1A^OE-GFP group. (f) The expression levels of c-jun, MMP2, and MMP9 in the HIF1A^OE-GFP group were higher than in Cherry-GFP group. The expression levels of c-jun, MMP2, and MMP9 in the HIF1A^OE-HMGN5^sh group were lower than in the HIF1A^OE-GFP group. Data were presented as the mean ± SD. ; ; .

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