Alleviation of Cartilage Destruction by Sinapic Acid in Experimental Osteoarthritis

Figure 2

Activation of heme oxygenase-1 (HO-1) expression and nuclear factor-erythroid 2-related factor 2 (Nrf2) by SA in primary mouse chondrocytes. (a) Cell viability assay. Primary mouse chondrocytes were treated with different doses of SA for 12 h. A cell counting kit (WST-8) was used to measure the living cells. The assays were repeated three times independently. (b) Primary mouse chondrocytes were treated with SA (0, 3, 9 μg/ml) for 12 h. The cell nuclear fractions of Nrf2 were tested by western blotting. (c) Quantification of nuclear Nrf2 from (b). p < 0.05, p < 0.01 compared with no-treatment control. (d) Transfected HEK293 cells were treated with 0 nM, 3μM, and 9 μg/ml of SA for 12 h and luciferase activity was measured. p<0.01 compared with no-treatment control. (e) Primary mouse chondrocytes were treated with various doses of SA (0, 1, 3, 9 μg/ml) for 12 h. HO-1 and Nrf2 were assayed by western blotting. (f) Primary mouse chondrocytes were treated with SA (9 μg/ml) for various times (0, 8, 16, 32 h), HO-1 and Nrf2 were assayed by western blotting.

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