Identification of Mitochondrial Ligands with Hepatoprotective Activity from Notopterygii Rhizoma et Radix Using Affinity Ultrafiltration/Liquid Chromatography/Mass Spectrometry

Table 1

LC/MS data and assignment of the four bioactive constituents in NRR extract.

NO.	tR (min)	∆P^b (%, n = 3)	UV λmax (nm)	[M + H]^+ [M + Na]^+m/z	ESI-MS^n (+) m/z (abundance)	[M − H]^+m/z	ESI-MS^n (-) m/z (abundance)	Predicted formula	Meas (m/z)	Pred (m/z)	Diff (ppm)	DBE	Assigned identification
^aP1	56.414	30.1 ± 3.5	250, 310	355.1537	—	353.1408	MS^2 (353): 353 (100), 254 (26), 297 (18), 210 (13), 214 (12), 269 (10)	C21H2205	353.1408	353.1358	4.67	11	Notopterol
^aP2	59.285	37.4 ± 4.2	295	301.1074	MS^2 (301): 233 (100)	299.2598	MS^2 (299): 229 (100), 69 (43)	C17H1605	301.1074	301.1414	1.16	9	Phellopterin
^aP3	60.857	28.3 ± 2.6	220, 250, 310	271.0969	MS^2 (271): 203 (100), MS^3 (203): 147 (73), 119 (38), 175 (10)	269.2127	MS^2 (269): 62 (100), 254 (49), 201 (29), 69 (26), 238 (16), 221 (15)	C16H1404	271.0969	271.0967	1.29	10	Isoimperatorin
P4	63.017	42.4 ± 5.1	230, 330	299.1102	MS^2 (299): 175 (100), 231 (12)	297.2427	—	C19H2203	298.1611	298.1867	4.65	9	7-Geranyloxycoumarin

^aComparison to standards. ^bΔP was calculated using ΔP = (P_e – P_c)/P_e × 100, where P_e and P_c are the peak areas in the experimental and control groups, respectively. Data were obtained from 3 independent experiments and are presented as the mean ± SD.