GYY4137 Attenuates Sodium Deoxycholate-Induced Intestinal Barrier Injury Both In Vitro and In Vivo

Figure 1

Destructive effects of SDC on Caco-2 monolayer barrier function and GYY4137 ameliorates intestinal epithelial barrier dysfunction induced by SDC. (a) Caco-2 monolayers were treated with different concentrations (0–2.4 mM) of SDC. 2 Mm or greater concentration could significantly decrease the TEER at 30 min. (b) Dose-response of the TEER of Caco-2 monolayers treated with different concentrations of SDC for 30 min. Concentration ≥2 mM significantly degraded the TEER. (c) Caco-2 monolayers were preincubated with or without 50, 100, and 200 μM GYY4137 for 48 h and then treated in the presence or absence of 2 mM SDC for 30 min. GYY4137 significantly attenuated TEER reduction induced by SDC treatment. (d) Caco-2 monolayers were treated as described. The increase of FD-40 flux induced by SDC was significantly attenuated by GYY4137 treatment. Results were expressed as mean ± SEM (n = 3). vs. control.

(a)

(b)

(c)

(d)