MiR-455-5p Suppresses the Progression of Prostate Cancer by Targeting CCR5

Figure 2

Biological functions of miR-455-5p on human PCa cell proliferation, cell apoptosis, and tumorigenicity. (a–c) Cell proliferation was assessed by CCK-8 and colony-formation assay in LNCAP and DU145 cells. (d) Cell apoptosis was analyzed by annexin V-FITC plus PI staining in both cells. (e) Western blot revealed the protein levels of caspase 3 and cleaved caspase 3 in LNCAP and DU145 cells 72 h after transfection. (f) After transfecting with LV-miR-455-5p virus, miR-455-5p expression was elevated compared with the negative-control group, as evaluated by qRT-PCR. (g) Image of tumor xenografts in nude mice injected subcutaneously with miR-455-5p-overexpressing DU145 cells. (h) Tumor volume was measured every 4 days. , P < 0.05.

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