Research Article
IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation
Table 1
The primers for this study.
| | Primers | Product length (pb) | ID | Nucleotide sequence of primers (5′–3′) |
| | Barrier function genes | | | | | Cldn1 | 107 | NM_001244539.1 | F: GCCACAGCAAGGTATGGTAAC | | R: AGTAGGGCACCTCCCAGAAG | | MYD88 | 108 | NM_010851.2 | F: GCATGGTGGTGGTTGTTTCTG | | R: GAATCAGTCGCTTCTGTTGG | | Ocln1 | 208 | NM_008756 | F: CCTACTCCTCCAATGGCAAA | | R: CTCTTGCCCTTTCCTGCTTT | | PepT1 | 99 | NM_214347.1 | F: CAGACTTCGACCACAACGGA | | R: TTATCCCGCCAGTACCCAGA | | Tjp1 m | 64 | XM_021187463.1 | F: ACCATCATTGTCGTCGCATGTAGATCC | | R: GATGCTCTAGGTGCCTGTTCGTAACG |
| | Cytokine genes | | | | | IL-1β | 87 | NM_001005149 | F: GAGCTGAAGGCTCTCCACCTC | | R: ATCGCTGTCATCTCCTTGCAC | | IL-4 | 382 | NM_021283.2 | F: GAGCCATATCCACGGATGCGACAA | | R: CATGGTGGCTCAGTACTACGAGTA | | IL-10 | 71 | XM_021175612.1 | F: GCCACATGCTCCTAGAGCTG | | R: CAGCTGGTCCTTTGTTTGAAA | | IL-17 | 119 | NM_010552.3 | F: TACCTCAACCGTTCCACGTC | | R: TTTCCCTCCGCATTGACAC | | IL-18 | 203 | XM_006510028.3 | F: AGACAACTTTGGCCGACTTC | | R: CCTTCACAGAGAGGGTCACA | | IL-22 | 73 | XM_006513865.3 | F: GCTCAGCTCCTGTCACATCA | | R: CACTGTCTCCTTCAGCCTTCT | | IL-23 | 194 | NM_031252.2 | F: AGTGTGAAGATGGTTGTGAC | | R: CTGGAGGAGTTGGCTGAG | | TLR4 | 64 | XM_006509283.3 | F: TTCAGAACTTCAGTGGCTGGATT | | R: CCATGCCTTGTCTTCAATTGTTT | | TNF-α | 192 | XM_021218154.1 | F: AGGCACTCCCCCAAAAGAT | | R: TGAGGGTCTGGGCCATAGAA | | IFN-g | 361 | NM_008337.4 | F: ATGAACGCTACACACTGCATCTTGGCTT | | R: CCTCAAACTTGGCAATACTCATGAATGC | | β-Actin | 79 | NM_007393.5 | F: AACGAGCGGTTCCGATGC | | R: GTAGTTCATGGATGCCACAGG |
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