Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition

Figure 6

TXL regulates EndMT through NRG-1/ ErbB signaling pathway in the presence of hypoxia. (a) Immunofluorescence for EndMT markers vWF and α-SMA in HCMECs treated with TXL and NRG-1 siRNA in the presence of hypoxia. Nuclear staining with DAPI (blue fluorescence). Scale bar: 50μm. (a) Effects of TXL on endothelial markers CD31 and VE-cadherin and mesenchymal markers α-SMA, FSP-1 and Vimentin with or without pretreatment with NRG-1 siRNA in the presence of hypoxia (1% O2) for 3 days. (b1)–(b5) showed corresponding column chart. n=3, P<0.01 vs. control group; ^# P<0.05 ^## P<0.01 vs. hypoxia group; P<0.05 P<0.01 vs. hypoxia +NRG-1 siRNA group; ⁺ P<0.05 ⁺⁺ P<0.01 vs. hypoxia +TXL group.

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