PTEN Inhibits Inflammatory Bone Loss in Ligature-Induced Periodontitis via IL1 and TNF-α

Figure 1

Ligature induced the periodontitis and inflammatory bone loss. (a) Histologic changes in periodontal tissue were monitored by hematoxylin and eosin (H&E) staining. Noticeable inflammatory reaction and damage to organizational structures were observed in ligature-induced periodontitis. Scale bars, 100 μm. (b) Higher magnification of (a). Scale bars, 50 μm. (c) Quantitative analysis of inflammation score of maxillary second molar periodontal tissues. Values are the mean ± SEM. Asterisks () denote significant differences (), n = 5 biological replicates. (d) TRAP staining showed a significant increase of TRAP-positive multinucleated osteoclasts in mouse maxilla in the ligature-induced periodontitis group. Scale bars, 100 μm. (e) Higher magnification of (d). Scale bars, 50 μm. (f) Quantitative analysis of TRAP-positive multinucleated osteoclasts showed a significantly increased number of osteoclasts in the ligature-induced periodontitis group compared to unligated group. N.Oc/B.Pm (mm⁻¹): number of osteoclasts per bone perimeter. Values are the mean ± SEM. , n = 5 biological replicates.

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