PTEN Inhibits Inflammatory Bone Loss in Ligature-Induced Periodontitis via IL1 and TNF-α

Figure 5

Forced overexpression of PTEN could inhibit inflammation and bone erosion. (a) Schematic figure shows PTEN nanoparticles (10 μg PTEN plasmids with nanoparticles) treated with the ligature on day 10 for two weeks, three times per week. (b) Levels of expression of mRNA for PTEN in periodontal tissue extracts obtained on day 24 from ligature and ligature with PTEN nanoparticle treatment mice. Data were normalized for GAPDH expression. Values are the mean ± SEM. ; n = 5 biological replicates. Relative mRNA expression levels for the inflammatory marker genes (c) IL1, (d) IL6, and (e) TNF-α in tissue extracts from periodontal tissue obtained as in (b). Values are the mean ± SEM. ; n = 5 biological replicates. Relative mRNA expression levels for the osteoclast marker genes (f) TRAP and (g) cathepsin K in tissue extracts from periodontal tissue obtained as in (b). Note that osteoclast marker genes decreased when treated with PTEN nanoparticles. Values are the mean ± SEM. ; ; n = 5 biological replicates.

(a)

(b)

(c)

(d)

(e)

(f)

(g)